解剖学报

›› 2011, Vol. 42 ›› Issue (6): 802-806.doi: 10.3969/j.issn.0529-1356.2011.06.017

• 肿瘤生物学 • 上一篇    下一篇

LGR5在肝癌细胞及肝癌组织中的表达及意义

宋竹雯1; 魏潇凡2; 高建超2; 白莉1*;张宏权2   

  1. 1.第三军医大学研究生大队, 重庆 400038; 2.北京大学医学部基础医学院解剖学与组织胚胎学系, 北京 100191
  • 收稿日期:2011-05-18 修回日期:2011-08-25 出版日期:2011-12-06
  • 通讯作者: 白莉

Expression of LGR5 in hepatoma cells, HCC tissue and its significance

  1. 1.Third Military Medical University Graduate Brigade, Chongqing 400038; 2.Department of Anatomy,Histology and Embryology,School of Basic Medical Sciences,Peking University, Beijing 100191,China
  • Received:2011-05-18 Revised:2011-08-25 Online:2011-12-06
  • Contact: BAI Li

关键词: LGR5, 肝癌, 免疫组织化学, 免疫印迹法, 实时荧光RT-PCR, 人

Abstract: Objective To investigate the expression of LGR5 in human hepatocelluar cancer tissues and four human liver cancer cell lines and to evaluate its value in the development of hepatocellular cancer.Methods The mRNA and protein expression of LGR5 in human liver cancer cell lines and normal liver cell were detected by real-time PCR and Western blotting. Expression of LGR5 and β-catenin in hepatocellular cancer tissue was determined by immunohistochemistry. LGR5 localization and expression in HepG2 cells were tested by immunocytochemical staining. Results Western blotting and real-time PCR results demonstrated that the expression of LGR5 decreased significantly in HepG2, Hep3B, Huh7 and PLC cells compared to LO2 cells(EM>P/EM> <0.05). Immunohistochemistry results also showed that LGR5 was downregulated in cancer tissue compared to adjacent tumor tissue, whereas β-catenin expression was upregulated in cancer tissue. Conclusion Compared to adjacent tumor tissue and normal liver cells, LGR5 expression was significantly downregulated in 36 cases of hepatocelluar cancer tissues and four kinds of h

Key words: LGR5, Hepatocelluar carcinoma, Immunohistochemistry, Western blotting, Real-time RT-PCR, Human

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